SIMULTANEOUS ESTIMATION OF PIPERINE, QUERCETIN, AND CURCUMIN IN A MIXTURE USING U.V-VISIBLE SPECTROPHOTOMETER AND METHOD VALIDATION.


Ginpreet. Aneja , Urja. Dave and K. Vadodaria

 U.V Spectrophotometric method have been widely employed in determination of individual components in a mixture or fixed dose combination. For the ternary mixture containing Quercetin, Curcumin and Piperine (Anti-inflammatory action) no spectrophotometric method for simultaneous evaluation has been reported so far. Thus our aim is to develop simultaneous equation method for estimation of the ternary mixture using U.V spectrophotometry. Methanol was used as solvent for dissolving each component and preparation of all the dilutions. The method involves formation and solving of simultaneous equations. Mixture of Quercetin, Curcumin and Piperine, having concentration 5.0 ppm, 5.0 ppm and 2.5 ppm, was subjected to overlay scan in U.V spectrophotometer from which λmax of same were found to be 371.31 nm, 424.68 nm, 343.76 nm respectively. Calibration curve depicted the concentration range following Beer Lambert’s law to be 5-30µg/ml, 1-5µg/ml and 1-10µg/ml respectively. Final concentration of Quercetin, Curcumin and Piperine in the mixture was found to be 5.3 ppm, 5.06 ppm, 2.25 ppm respectively. Accuracy and precision were also assessed in order to validate the method. 
KEYWORDS: Quercetin, piperine, curcumin, simultaneous estimation, spectrophotometric method.