THE EFFECT OF VITAMIN E IN THE GENTAMICIN INDUCED NEPHROTOXICITY IN WISTAR ALBINO RATS- A HISTOLOGICAL PERSPECTIVE


Yadav G, Awasthi J R, Pandey N, Shrestha S, Jha CB

ABSTRACT

Introduction: Gentamicin is commonly applied aminoglycoside antibiotic for the treatment of gram negative infection which causes nephrotoxicity. Vitamin E had shown promising effect in Cisplatin and adriamycin-induced nephrotoxicity and expected to show the palliative effect in GM- induced nephrotoxicity.

Aims & Objectives: To investigate whether Vitamin E treatment prevents gentamicin-induced nephrotoxicity. Materials and Methods: 32 healthy Wistar albino rats of either sex weighing 150-200 gm were randomly selected

and divided into four groups, each consisting of eight rats. Group I received 1mL of saline intraperitoneally (i.p), group II received Vitamin E 200 mg/kg i.p., group III received GM 100 mg/kg i.p. and group IV was given Vitamin E 200 mg/kg i.p. before being given the same dose of GM as the group II daily in single dose throughout 8 days of experiment. On 9th day rats were sacrificed, both kidneys from each rat were removed and kept in 10% formalin and histological analysis was done.

Results: Examination of renal cortex showed normal architecture in group I and II. GM treated rats showed dilated tubules and glomerulus with cell desquamation, patchy necrosis, and the presence of cellular debris with some desquamated epithelial cells in their lumen. GM with vitamin E treated rats showed almost normal glomeruli, proximal convoluted tubules and distal convoluted tubules. However there were areas of interstitial hemorrhage and vascular congestion suggesting that the vitamin E offer only partial protective effect.

Conclusion: Vitamin E was partially effective in reversing the GM induced nephrotoxicity in Wistar albino rats. Key Words: Vitamin E, nephrotoxicity, gentamicin, kidney